We take pride in creating high-quality short-wave pass edge filters, suitable for a wide range of applications including medical, scientific research, and defense. Our filters are typically made of optical glass substrates with multiple layers of dielectric films, offering excellent light transmission capabilities for UV, visible, and IR applications. These high-transmission filters are designed for robust performance under various conditions, demonstrating outstanding durability and the ability to withstand normal handling and cleaning processes.
I. Product Functions
As a core component of the fluorescence detection system, our company's fluorescence filters achieve three key functions: precisely screening excitation light, efficiently separating excitation light from fluorescence signals, and purifying the emitted light while blocking stray light, ensuring high purity and high signal-to-noise ratio of the detection signal. Our product range includes bandpass (BP), longpass (LP), shortpass (SP), and multi-bandpass filters, and can be customized to fit specific fluorescent dyes and detection equipment.
II. Working Principle
Based on the principle of optical thin film interference, the filter set purifies the signal in three steps: 1. The excitation filter only allows the target excitation light to pass through and illuminate the sample for detection; 2. The dichroic mirror reflects the excitation light and transmits the fluorescence emitted by the sample, achieving the separation of the optical path; 3. The emission filter blocks the residual excitation light and background noise, outputting a pure fluorescence signal to ensure the accuracy of the detection.
III. Production and Manufacturing
Craftsmanship builds quality, highlighting the strength of intelligent manufacturing. We are well aware that quality is the lifeline of an enterprise and a direct manifestation of its strength. Therefore, we have established an all-inclusive intelligent manufacturing system integrating "research and development, production, and testing", controlling every detail from the source: carefully selecting high-purity optical glass or quartz to prepare the substrate, and ensuring surface flatness through precise cutting, grinding, and polishing; adopting advanced vacuum coating processes such as electron beam evaporation and ion-assisted deposition to precisely control the thickness of the film layer; and equipping with top-notch testing equipment for strict control throughout the process to ensure that each product meets the industry's high standards.
IV. Application Fields
Relying on stable and reliable product performance, it is widely applied in core scenarios of multiple fields: life science and medical care (fluorescence microscopes, qPCR instruments, flow cytometers, POCT rapid diagnosis), industry and scientific research (Raman spectrometers, machine vision inspection, environmental monitoring, biochips), and special scenarios (anti-counterfeiting detection, LED display color purity correction).
Ultraopto’s Fluorescence Filters are a series of high-performance optical filters specially engineered for fluorescence imaging and detection applications, including excitation filters, emission filters and dichroic beam splitters, designed to work in tandem to isolate specific excitation and emission wavelengths, maximize fluorescence signal transmission and minimize background noise, serving as core components in fluorescence microscopy, flow cytometry, confocal imaging and biomedical diagnostic systems. Crafted from premium optical-grade glass and fused silica substrates with advanced high-vacuum ion beam sputtering coating technology, our Fluorescence Filters deliver an average peak transmittance of ≥90% for the target excitation and emission bands, with steep spectral edges and narrow passband widths that ensure precise spectral isolation of fluorescence signals, and the rejection region features an ultra-deep optical density of ≥OD6, effectively blocking unwanted excitation light and stray light, maximizing the signal-to-noise ratio and achieving high-contrast fluorescence imaging for even the weakest biological fluorescence signals. Each set of Fluorescence Filters is precisely matched for center wavelengths, passband widths and spectral edges, ensuring perfect spectral compatibility and no mutual interference between excitation, emission and dichroic beam splitter filters.
We implement strict precision optical processing and coating quality control for all Fluorescence Filter products, achieving a superior surface quality rating of 60/40 scratch-dig and a clear aperture of over 90%, minimizing light scattering and reflection loss for optimal fluorescence signal transmission, and the filters are manufactured with ultra-strict center wavelength tolerances to ensure precise matching with common fluorescence dyes and probes (FITC, GFP, RFP, Cy3, Cy5, etc.). The durable hard coatings exhibit excellent environmental stability and mechanical resistance, with scratch resistance, temperature change resistance, humidity resistance and chemical corrosion resistance, ensuring long-term stable operation in both laboratory precision fluorescence microscopy and clinical/industrial field conditions, and the filters have a long service life with no significant spectral performance degradation even after frequent cleaning and use.
Our Fluorescence Filters are available in both standard and custom sizes, with compact and thin structural designs that enable seamless integration into compact fluorescence imaging systems, portable detection devices and miniature biomedical diagnostic instruments, and we also offer custom coating designs for special fluorescence dyes and probes with non-standard excitation/emission wavelengths.
Ultraopto’s Fluorescence Filters are widely applied in all professional fluorescence optical fields, including fluorescence microscopy for high-contrast imaging of biological samples such as cells, tissues and microorganisms, enabling biomedical research on cell structure, molecular interactions and disease mechanisms, and in flow cytometry for high-throughput analysis and sorting of fluorescently labeled cells, achieving accurate diagnosis of hematological diseases, cancer and immune system disorders. In confocal laser scanning microscopy, they are essential components for high-resolution 3D fluorescence imaging of biological samples, and in in vivo fluorescence imaging for non-invasive monitoring of biological processes and disease progression in living organisms for preclinical research and drug development. They also serve as core components in biomedical diagnostic instruments for fluorescence-based clinical testing such as immunoassays and nucleic acid detection, in environmental monitoring for detecting fluorescently labeled pollutants and microorganisms, and in industrial quality control for detecting fluorescently labeled defects in industrial products such as semiconductors and display panels. Additionally, the filters are used in academic research and fluorescence optical experiment platforms for studying fluorescence characteristics of new materials and developing new fluorescence dyes and probes.
As a leading manufacturer of high-performance fluorescence optical filters, Ultraopto offers both standard and fully customized Fluorescence Filter solutions to meet your unique fluorescence imaging and detection requirements. Our professional R&D and engineering team, with rich expertise in fluorescence spectroscopy and optical filter design, can provide standard filter sets matched for common fluorescence dyes and probes (FITC, GFP, RFP, Cy3, Cy5, etc.), and also customize excitation filters, emission filters and dichroic beam splitters for special non-standard fluorescence dyes and probes, tailoring the center wavelength, passband width, transmittance and optical density level to ensure optimal fluorescence signal isolation and detection. We can customize the filter dimensions, thickness and mounting options to fit your specific fluorescence imaging system integration needs, as well as custom anti-reflection coatings to further reduce surface reflection loss and enhance fluorescence signal transmittance. All custom
Fluorescence Filter orders undergo multi-stage strict fluorescence optical performance testing and quality inspection, including transmittance measurement of excitation/emission bands, optical density testing, spectral matching verification and environmental stability testing, with a detailed quality inspection report provided to ensure the final product fully complies with your design specifications and application needs. Backed by advanced ion beam sputtering coating technology, a complete quality control system and stable batch supply capacity, Ultraopto is committed to providing global customers with high-quality Fluorescence Filter products and one-stop fluorescence optical solutions. For more detailed technical parameters, product quotation information or custom design solutions for our Ultraopto Fluorescence Filters, please contact our professional sales and technical team through the official website, and we will provide you with efficient, professional and personalized optical solutions to support the development and production of your fluorescence imaging and diagnostic systems.
